Erk1/2 Antibody: ATTO 488-多抗-深圳欣博盛生物科技有限公司

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Erk1/2 Antibody: ATTO 488

品牌：Stressmarq

官网链接

说明书

折扣/货期

货号：SPC-120D-A488

规格：100 µl

目录价：￥4296.6

产品详情

* 以下信息仅供参考，详情请以原厂网站为准

产品名称:

Erk1/2 Antibody: ATTO 488

别名:

ERK1 Antibody, ERK2 Antibody, ERT1 Antibody, ERT2 Antibody, MAP kinase1 Antibody, MAP kinase2 Antibody, MAPK1 Antibody, MAPK2 Antibody, MAPK3 Antibody, p38 Antibody, p40 Antibody, p41 Antibody, p41mapk Antibody, p42 MAPK Antibody, p44 ERK1 Antibody, p44 MAPK Antibody, PRKM1 PRKM2 Antibody, PRKM3 Antibody

产品描述:

Rabbit Anti-Rat Erk1/2 Polyclonal

反应种属:

宿主来源:

Rabbit

实验应用:

WB | IHC | ICC/IF | FCM

靶标/特异性:

Detects ~44kda (ERK1) and ~42kDa (ERK2).

推荐稀释度:

WB (1:1000), IHC (1:100), ICC/IF (1:100), FCM (1:100); optimal dilutions for assays should be determined by the user.

免疫原:

A 35 residue synthetic peptide, corresponding to Rat Erk1 MAP kinase with the CGG spacer group added and the peptide coupled to KLH.

免疫原种属:

Rat

克隆性:

Polyclonal

纯化方式:

Peptide Affinity Purified

偶联:

ATTO 488

产品浓度:

1 mg/ml

保存温度:

Conjugated antibodies should be stored according to the product label

运输温度:

Blue Ice or 4ºC

存储溶液:

PBS pH7.4, 50% glycerol, 0.09% sodium azide *Storage buffer may change when conjugated

产地:

加拿大

功能与背景:

The extracellular signal-regulated kinases 1 and 2 (ERK1 and ERK2), also called p44 and p42 MAP kinases, are members of the Mitogen Activated Protein Kinase (MAPK) family of proteins found in all eukaryotes. Because the 44 kDa ERK1 and the 42 kDa ERK2 are highly homologous and both function in the same protein kinase cascade, the two proteins are often referred to collectively as ERK1/2 or p44/p42 MAP kinase (1). They are both located in the cytosol and mitochondria (2). While the role of cytosol ERK1/2 is well studied and involved in multiple cellular functions (2), the role of mitochondrial ERK1/2 remains poorly understood. Both ERK 1 and 2 are activated by MEK1 or MEK2, by dual phosphorylation of a threonine and tyrosine residue in the activation loop (TEY motif) (1, 3). Either phosphorylation alone can induce an electrophoretic mobility shift, but both are required for activation of the kinase. This dual phosphorylation is efficiently detected by phosphorylation state-specific antibody directed to the pTEpY motif. Once activated, MAP kinases phosphorylate a broad spectrum of substrates, including cytoskeletal proteins, translation regulators, transcription factors, and the Rsk family of protein kinases (4). ERK1/2 activation is generally thought to confer a survival advantage to cells (5); however there is increasing evidence that suggests that the activation of ERK1/2 also contributes to cell death under certain conditions (5). ERK1/2 also is activated in neuronal and renal epithelial cells upon exposure to oxidative stress and toxicants or deprivation of growth factors, and inhibition of the ERK pathway blocks apoptosis (5).

Accession #:

NP_059043.1