Human Recombinant Alpha Synuclein A90C Mutant Monomers-其他重组蛋白-深圳欣博盛生物科技有限公司

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Human Recombinant Alpha Synuclein A90C Mutant Monomers

品牌：Stressmarq

官网链接

说明书

折扣/货期

货号：SPR-478B

规格：100 µg

目录价：￥5418

产品详情

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产品名称:

Human Recombinant Alpha Synuclein A90C Mutant Monomers

别名:

SNCA, alpha-synuclein, synuclein, Alpha synuclein monomer, Alpha-synuclein monomer, Alpha synuclein protein monomer, Alpha synuclein monomer, Alpha-synuclein protein, SNCA protein

产品描述:

Human Recombinant Alpha Synuclein A90C Mutant Monomers

反应种属:

Human

实验应用:

WB | SDS PAGE | In vitro Assay | Conjugation | FRET

纯化方式:

Ion-exchange Purified

偶联:

No Tag

产品浓度:

2 mg/ml

表达系统:

E. coli

保存温度:

-80ºC

运输温度:

Dry Ice. Shipping note: Product will be shipped separately from other products purchased in the same order.

预期分子量:

14.49 kDa

存储溶液:

20mM Hepes pH 7.4, 150mM NaCl, 1mM TCEP pH 7.0

产地:

加拿大

功能与背景:

Thioflavin-T (ThT) fluorescence remains a common measurement of alpha-synuclein fibril formation, yet ThT exhibits poor affinity for oligomers and early aggregates. The alpha-synuclein A90C mutant monomers can be specifically labelled with alternative fluorophores (such as Alexa 488/Alexa 647) via maleimide chemistry to enable more sensitive FRET analysis of aggregation. The A90C mutant showed no perturbation of monomer structure and Alexa Fluor dye attachment to cysteine 90 was demonstrated to have no effect on the kinetics of fibril formation (1-3). Residue 90 is at the periphery of the NAC region, a key constituent of the alpha-synuclein β-sheet fibril core, which results in fluorophores on different monomers coming into close proximity upon formation of β-sheet structure during aggregation (4). Note - to prevent the potential mis-translation of alpha-synuclein Y136 as C136 during E.coli expression, the Y136-TAT construct was used (5).

纯度:

>95%