Human Recombinant Alpha Synuclein E114C Mutant Pre-formed Fibrils: ATTO 488-其他重组蛋白-深圳欣博盛生物科技有限公司

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Human Recombinant Alpha Synuclein E114C Mutant Pre-formed Fibrils: ATTO 488

品牌：Stressmarq

官网链接

说明书

折扣/货期

货号：SPR-518E-A488

规格：100 µg x5

目录价：￥23751

产品详情

* 以下信息仅供参考，详情请以原厂网站为准

产品名称:

Human Recombinant Alpha Synuclein E114C Mutant Pre-formed Fibrils: ATTO 488

别名:

Alpha synuclein pre-formed fibril, Alpha-synuclein PFF, Alpha synuclein protein fibrils, Alpha-synuclein protein, Non-A beta component of AD amyloid protein, Non-A4 component of amyloid precursor protein, NACP protein, SNCA protein, NACP protein, PARK1 protein, SYN protein, Parkinson's disease familial 1 Protein

产品描述:

Human Recombinant Alpha Synuclein E114C Mutant Pre-formed Fibrils: ATTO 488

反应种属:

Human

实验应用:

WB | Native PAGE | In vitro Assay | In vivo Assay

纯化方式:

Ion-exchange & SEC purified

偶联:

ATTO 488

产品浓度:

Lot/batch specific. See included datasheet.

表达系统:

E.coli

保存温度:

-80ºC

运输温度:

Dry Ice. Shipping note: Product will be shipped separately from other products purchased in the same order.

预期分子量:

14.434 kDa

存储溶液:

1X PBS pH 7.4

产地:

加拿大

功能与背景:

The alpha-synuclein (aSyn) E114C mutation facilitates a single site-specific conjugation with ATTO-488 maleimide that avoids any hindrance on fibrilization or cell entry that may be conferred by non-specific lysine targeting conjugations. This conjugation is ideal due to internal position relative to C-terminal truncation sites, proximity to the NAC, and lack of interference with recruitment in vitro or in primary neurons (1, 2). Pre-formed fibrils (PFFs) generated with 5-25% fluorescently tagged E114C mutants have demonstrated a relative potency >80% compared to wild-type aSyn for inducing misfolding of endogenous aSyn, indicating no significant perturbation of seeding in living cells (1). Atto-488 is a useful tool for identifying cell entry, as the addition of Trypan Blue to cultures prior to imaging will quench fluorescence of extracellular Atto-488 conjugated aSyn (3). Our aSyn E114C-Atto-488 PFFs, which are formed from 10% fluorescently tagged E114C mutants and 90% wild-type monomers, are an excellent tool for studying cell entry and localization, with demonstrated entry into neurons after trypan blue quenching.

纯度:

>95%