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折扣/货期| 货号：97-099 | 规格：0.025 mg | 目录价：￥10933.65 |
产品详情
* 以下信息仅供参考，详情请以原厂网站为准
产品名称:
PH20 Hyaluronidase Recombinant Protein, Tag Free
别名:
SPAM1, PH-20, HYAL3, HYA1, HYAL1, HYAL5, SPAG15
实验应用:
WB
表达系统:
HEK293 cells
保存温度:
Lyophilized Protein should be stored at -20˚C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20˚C or -70˚C. Avoid repeated freeze-thaw cycles.
运输温度:
Blue Ice
产品形式:
Lyophilized
存储溶液:
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM NaCl, pH7.5 with trehalose as protectant.
产地:
美国
生产商:
Prosci
功能与背景:
Hyaluronidase PH-20 is also known as Sperm adhesion molecule 1 (SPAM1) and Sperm surface protein PH-20, which belongs to the glycosyl hydrolase 56 family, SPAM1 / PH-20 is expressed in testis. SPAM-1 / PH20 random hydrolysis of (1->4)-linkages between N – acetyl – beta – D – glucosamine and D-glucuronate residues in hyaluronate. SPAM-1 / PH20 involved in sperm-egg adhesion. Upon fertilization sperm must first penetrate a layer of cumulus cells that surrounds the egg before reaching the zona pellucida. The cumulus cells are embedded in a matrix containing hyaluronic acid which is formed prior to ovulation. SPAM1 aids in penetrating the layer of cumulus cells by digesting hyaluronic acid.
纯度:
>95% as determined by SDS-PAGE and >95% as determined by SEC-MALS.
生物活性:
The activity of PH20 is measured by its ability to hydrolyze HA in turbidimetric assay (45 minute assay). The specific activity is >55000 U/mg. (Unit Definition: One unit of Hyaluronidase activity will cause a change in A600 of 0.330 per minute at pH5.35 at 37 ˚C in a 2.0 mL reaction mixture) (QC tested).
Accession #:
AAH26163
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