Rab4 Antibody: ATTO 390-多抗-深圳欣博盛生物科技有限公司

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Rab4 Antibody: ATTO 390

品牌：Stressmarq

官网链接

说明书

折扣/货期

货号：SPC-141D-A390

规格：100 µl

目录价：￥4334.4

产品详情

* 以下信息仅供参考，详情请以原厂网站为准

产品名称:

Rab4 Antibody: ATTO 390

别名:

Oncogene Rab4 Antibody, Rab4A Antibody, Ras related protein Antibody

产品描述:

Rabbit Anti-Human Rab4 Polyclonal

反应种属:

Human | Mouse | Rat

宿主来源:

Rabbit

实验应用:

WB | IHC | ICC/IF

靶标/特异性:

Detects ~26kDa.

推荐稀释度:

WB (1:1000), IHC (1:100), ICC/IF (1:150); optimal dilutions for assays should be determined by the user.

免疫原:

C-terminal peptide from human Rab4

免疫原种属:

Human

克隆性:

Polyclonal

纯化方式:

Peptide Affinity Purified

偶联:

ATTO 390

产品浓度:

1 mg/ml

保存温度:

Conjugated antibodies should be stored according to the product label

运输温度:

Blue Ice or 4ºC

存储溶液:

PBS pH7.4, 50% glycerol, 0.09% sodium azide *Storage buffer may change when conjugated

产地:

加拿大

功能与背景:

Rab4 is a 25kDa member of the Rab family of small guanosine triphosphatases (GTPases), Ras superfamily. Rab GTPases are central regulators of membrane trafficking in the eukaryotic cell. Their regulatory capacity depends on their ability to cycle between the GDP -bound inactive and GTP-bound active states. This conversion is regulated by GDP/GTP exchange factors (GEPs), GDP dissociation inhibitors (GDIs) and GTPase-activating proteins (GAPs) (1, 2). Activation of a Rab protein is coupled to its association with intracellular membranes, allowing it to recruit downstream effector proteins to the cytoplasmic surface of a sub-cellular compartment (3). Through these proteins, Rab GTPases regulate vesicle formation, actin- and tubulin-dependent vesicle movement, and membrane fusion(1). Rab proteins contain conserved regions involved in guanine-nucleotide binding, and hyper-variable COHO-terminal domains with a cysteine motif implicated in sub-cellular targeting. Post-translational modification of the cysteine motif with one or two geranylgeranyl groups is essential for the membrane association and correct intracellular localization of Rab proteins (3). Each Rab shows a characteristic sub-cellular distribution (4). In particular, over-expression of Rab4 causes a redistribution of receptors on plasma membrane versus endocytic compartments. The presence of excessive Rab4 leads to the accumulation of tranferrin receptors in non-acidic, post-endosomal recycling vesicles considered an intermediate compartment between endosomes and plasma membranes. Rab4 also plays a role in the translocation of glucose transporter (Glu4) in adipocytes in response to insulin (5). Mediating the association of Rab4 with transferring receptor-containing early endosomes takes place through the geranylgeranyl groups at its carboxyl-terminus. Membrane association is also cell cycle dependent, as phosphorylation at its c-terminal cdc2 kinase consensus sequence in mitotic cells leads to dissociation of Rab4 into the cytosol (6).

Accession #:

NP_004569.2