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折扣/货期| 货号：40-575-0.005 mg | 规格：0.005 mg | 目录价：￥3783 |
| 货号：40-575-0.025 mg | 规格：0.025 mg | 目录价：￥5674.5 |
产品详情
* 以下信息仅供参考，详情请以原厂网站为准
产品名称:
TIGAR-TAT Recombinant Protein
别名:
FR2BP, TIGAR, C12orf5, Fructose-2, 6-bisphosphatase TIGAR, TP53-induced glycolysis and apoptosis regulator
表达系统:
E. coli
保存温度:
The lyophilized TIGAR-TAT recombinant protein is stable for at least 2 years from date of receipt at -20˚C. Reconstituted TIGAR-TAT stable for at least 3 months when stored in working aliquots with a carrier protein at -20˚C. As with any protein, exposing TIGAR-TAT recombinant protein to repeated freeze / thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.
运输温度:
Blue Ice
产品形式:
Lyophilized
产地:
美国
生产商:
Prosci
功能与背景:
TIGAR is a p53-inducible enzyme that catalyzes the hydrolysis of fructose-2-6 bisphosphate (F-2-6-BP) to fructose-6-phosphate and inorganic phosphate. F-2-6-BP is a powerful activator of 6-phosphofructose-1 kinase, the rate limiting enzyme of glycolysis. By lowering the intracellular level of F-2-6-BP, TIGAR expression leads to increased glucose processing via the pentose phosphate pathway, the major cellular source for NADPH. Protein transduction using TAT fusion proteins represents an alternative methodology for introducing transcription factors and other intracellular proteins into primary as well as transformed cells. Recombinant human TIGAR-TAT expressed in E. coli is a 36 kDa protein containing 284 amino-acid residues, including the 271 residues of full-length TIGAR fused to a 13-residue C-terminal peptide containing the TAT transduction domain (GGYGRKKRRQRRR).
纯度:
Greater than 95% by SDS-PAGE gel and HPLC analyses.
生物活性:
Pretreatment with TIGAR-TAT for 4 hrs, using a concentration range 0.1-5.0 μg/mL, protects U2OS cells from apoptosis induced by hydrogen peroxide. Endotoxin level is less than 0.1 ng per μg (1EU/μg).
Accession #:
NP_065108.1
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