Ubiquitin Antibody: APC-单抗-深圳欣博盛生物科技有限公司

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Ubiquitin Antibody: APC

品牌：Stressmarq

官网链接

说明书

折扣/货期

货号：SMC-171B-APC

规格：200 µg

目录价：￥5594.4

产品详情

* 以下信息仅供参考，详情请以原厂网站为准

产品名称:

Ubiquitin Antibody: APC

别名:

Polyubiquitin B Antibody, RPS27A Antibody, UBA52 Antibody, UBB Antibody, UBC Antibody, ubiquitin B Antibody

产品描述:

Mouse Anti-Bovine Ubiquitin Monoclonal IgG2a Kappa

反应种属:

Human | Mouse | Rat | Bovine

宿主来源:

Mouse

实验应用:

WB | ICC/IF | ELISA

靶标/特异性:

Detects ~10kDa.

同种型:

IgG2a Kappa

推荐稀释度:

WB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.

免疫原:

Native bovine ubiquitin, conjugated to KLH

免疫原种属:

Bovine

克隆性:

Monoclonal

克隆号:

6C11-B3

纯化方式:

Protein G Purified

偶联:

APC

产品浓度:

1 mg/ml

保存温度:

Conjugated antibodies should be stored according to the product label

运输温度:

Blue Ice or 4ºC

存储溶液:

PBS pH7.4, 50% glycerol, 0.09% sodium azide *Storage buffer may change when conjugated

产地:

加拿大

功能与背景:

Ubiquitin is a small protein that occurs in all eukaryotic cells. The ubiquitin protein itself consists of 76 amino acids and has a molecular mass of about 8.5kDa. Key features include its C-terminal tail and the 7 Lys residues. It is highly conserved among eukaryotic species: Human and yeast ubiquitin share 96% sequence identity (1). The main function of Ubiquitin is to clear abnormal, foreign and improperly folded proteins by targeting them for degradation by the 26S proteosome (2). Ubiquitination represents an essential cellular process affected by a multi-enzyme cascade involving classes of enzymes known as ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s or Ubcs) and ubiquitin-protein ligases (E3s). Ubiquitin is activated in a two-step reaction by an E1 ubiquitin-activating enzyme in a process requiring ATP as an energy source. The initial step involves production of an ubiquitin-adenylate intermediate. The second step transfers ubiquitin to the E1 active site cysteine residue, with release of AMP. This step results in a thioester linkage between the C-terminal carboxyl group of ubiquitin and the E1 cysteine sulfhydryl group. The third step is a transfer of ubiquitin from E1 to the active site cysteine of a ubiquitin-conjugating enzyme E2 via a trans(thio)esterification reaction. And the final step of the ubiquitylation cascade creates an isopeptide bond between a lysine of the target protein and the C-terminal glycine of ubiquitin. In general, this step requires the activity of one of the hundreds of E3 ubiquitin-protein ligases (often termed simply ubiquitin ligase). E3 enzymes function as the substrate recognition modules of the system and are capable of interaction with both E2 and substrate(2, 3). Ubiquitination also participates in the internalization and degradation of plasma membrane proteins such as some of the TCR subunits while still ER-membrane associated (4). Ubiquitin also plays a role in regulating signal transduction cascades through the elimination inhibitory proteins, such as IκBα and p27 (5).

Accession #:

NP_776558.1