HIF1 alpha Antibody: RPE-单抗-深圳欣博盛生物科技有限公司

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HIF1 alpha Antibody: RPE

品牌：Stressmarq

官网链接

说明书

折扣/货期

货号：SMC-184D-RPE

规格：100 µg

目录价：￥4422.6

产品详情

* 以下信息仅供参考，详情请以原厂网站为准

产品名称:

HIF1 alpha Antibody: RPE

别名:

ARNT interacting protein Antibody, HIF1A Antibody, Hypoxia inducible factor 1 alpha Antibody, MOP1 Antibody, PASD8 Antibody

产品描述:

Mouse Anti-Mouse HIF1 alpha Monoclonal IgG1

反应种属:

Human | Mouse | Rat | Bovine

宿主来源:

Mouse

实验应用:

WB | IHC | ICC/IF | ELISA

靶标/特异性:

Detects ~116kDa. Specific for HIF1Alpha.

同种型:

IgG1

推荐稀释度:

WB (1:1000), IHC (1:100), ICC/IF (1:50); optimal dilutions for assays should be determined by the user.

免疫原:

Mouse recombinant fragment corresponding to amino acids 329-542

免疫原种属:

Mouse

克隆性:

Monoclonal

克隆号:

ESEE122

纯化方式:

Protein G Purified

偶联:

RPE

产品浓度:

1 mg/ml

保存温度:

Conjugated antibodies should be stored according to the product label

运输温度:

Blue Ice or 4ºC

存储溶液:

PBS pH7.4, 50% glycerol, 0.09% sodium azide *Storage buffer may change when conjugated

产地:

加拿大

功能与背景:

Hypoxia-inducible factor 1 (HIF1) is a heterodimeric transcription factor that plays a critical role in the cellular response of hypoxia (1). The HIF1 complex consists of two subunits, HIF1-Alpha and HIF1-Beta, which are basic helix-loop-helix proteins of the PAS family (2). HIF1 regulates the transcription of a broad range of genes that facilitate responses to the hypoxic environment, including genes regulating angiogenesis, erythropoiesis, cell cycle, metabolism and apoptosis. The widely expressed HIF-1α is typically degraded rapidly in normoxic cells by the ubiquitin/proteasomal pathway. Under normoxic conditions, HIF-1α is proline hydroxylated leading to a conformational change that promotes binding to the von Hippel Lindau protein (VLH) E3 ligase complex; ubiquitination and proteasomal degradation follows (3, 4). Both hypoxic conditions and chemical hydroxylase inhibitors (such as desferrioxamine and cobalt) inhibit HIF-1α degradation and lead to its stabilization. In addition, HIF-1α can be induced in an oxygen-independent manner by various cytokines through the PI3K-AKT-mTOR pathway (5-7).

Accession #:

NP_034561.2